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2018-03-23至2018-03-24 上海
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肖华 教授

上海交通大学生命科学与技术学院

Comparative proteomic analysis of circulating tumor exosomes in human saliva and serum for lung cancer

肖华 教授

演讲题目:Comparative proteomic analysis of circulating tumor exosomes in human saliva and serum for lung cancer

上海交通大学生命科学与技术学院

肖华博士,上海交通大学研究员,博士生导师,国家青年千人,科技部青年科学家。毕业于中国科学院大连化学物理研究所,获分析化学博士学位。曾先后在美国加州大学欧文分校、加州大学洛杉矶分校和密西根大学安娜堡分校从事研究工作。现任上海千人计划专家联谊会大健康专委会执委、人类蛋白质组组织会员、中国蛋白质组学专业委员会委员、青年学者委员会委员、上海市科委自然科学基金评审专家。先后在国际重要学术期刊发表论文40余篇;申请发明专利10余项。先后主持多项科技部和基金委项目,并承担国家重点研发计划任务课题等。近年来主要围绕生物分离分析和癌症的分子诊断开展研究,研究方向包括生物分析新技术和转化医学、蛋白质组学和蛋白质翻译后修饰、疾病生物标志物的发现与验证等。发展了复杂蛋白质分离新理论新技术;建立了体液和细胞外泌体分离分析新方法;开拓了无创癌症分子诊断新策略。

演讲题目:Comparative proteomic analysis of circulating tumor exosomes in human saliva and serum for lung cancer

演讲摘要:Tumor-derived exosomes harbor plenty of cancer biological information, which has emerged as promising targets for cancer early detection and treatment. Human serum and saliva are unique diagnostic body fluids, which contain numerous circulating exosomes. Salivary and serum exosomes were enriched through commercial kit after high abundant protein removal. They were further confirmed by morphology analysis, nanoparticle tracking analysis, and surface biomarker test. Label free quantification was applied to systematically compare the protein profiling in salivary and serum exosomes. Their similarities and differences were systematically compared for the first time. 319 and 994 exosomal proteins were identified from saliva and serum by LC-MS/MS, respectively. Around 80% of salivary exosomal proteins were shared with serum exosomes. To explore their utility for cancer proteomics, we systematically compared the proteome of salivary and serum exosomes from healthy subjects and lung cancer patients. In particular, 11 potential candidates were coincidently discovered in both body fluids of lung cancer patients. Selected 6 exosomal protein candidates were further verified and evaluated through immunoassay. We further isolated microvesicles and exosomes in human saliva and comprehensively analyzed their proteome. 40 and 25 potential proteins originally from distal cells were found in the circulating salivary microvesicles and exosomes of lung cancer subjects. Our finding enforced the hypothesis that cancer related proteins were presented in saliva and serum exosomes, which promoted the unique features of exosomes in our body fluids. A circulating exosomes based body fluid test could be easily established for monitoring cancer once these candidates were validated.


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